| ZAG

Zinc-alpha-2-glycoprotein is a protein that is encoded by the AZGP1 gene. AZGP1, also referred to as zinc-alpha-2-glycoprotein (ZAG), is a glycoprotein with a molecular weight of 38-40 kDa. In 1961, AZGP1 was initially isolated from normal human plasma and named as ZAG due to its distinctive electrophoretic mobility within the alpha-2 region and its ability to bind to zinc. Subsequent analysis further identified its specific location on a particular chromosome on chromosome 7q22.1 by fluorescence in situ hybridization. AZGP1 is widely expressed in various tissues and body fluids, including the breast, stomach, liver, prostate, plasma, urine, and saliva.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	80	40	20	10	5	2.5	1.25	0
OD	1.98	1.1	0.71	0.49	0.31	0.21	0.17	0.07
Corrected OD	1.91	1.03	0.64	0.42	0.24	0.14	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse ZAG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Mouse ZAG and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-97
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98